Procedure for elution

The procedures:

  • First the generator will be removed from its shipping container (unwrapping) and placed in an appropriate area in the laboratory.  

A sterile isotonic saline solution must be provided for the elution step. In most designs the eluent will be obtained from a standard septum vial by attaching the vial to the generator using the inlet spike provided for this purpose. In this latter design there will be a set procedure to maintain sterility of the system, which will involve wiping the elution vial with an alcohol swab and allowing it to dry, then uncovering the inlet spike on the generator and attaching the vial by piercing the spike into the vial.

  • A sterile elution vial is prepared.  The eluate vial is placed in a suitable shield to absorb the radiation from the 99mTc activity. The septum is wiped with an alcohol swab and allowed to dry.

Removing a protective cap exposes the elution port and generally a fresh sterile needle is placed on the elution port for this and each subsequent elution. The elution vial in its shield is then pushed down onto the elution needle fully piercing the septum. The vacuum in the elution vial causes the saline to be drawn through the generator column thereby eluting the 99mTc activity.

The elution vial in its shield is removed from the generator and the elution needle can be capped or protected or replaced with a fresh sterile needle. If the generator uses an eluent vial this will be left on the inlet needle until the next elution.

  • The vial is taken for activity assay and quality control tests of the eluted 99mTc before being released for dispensing or use in the preparation of various 99mTc radiopharmaceuticals.

Adequate radiation shielding must be maintained at all times.

Based on IAEA Human Health Campus

Last modified: Monday, 18 March 2019, 5:04 AM